According to the Technical Guidance Document on Risk Assessment (Part II), C. elegans is a possible alternative test organism to refine the PNEC of biocides for the soil compartment. For the sediment compartment, besides tests with Lumbriculus variegatus and chironomids, „long-term tests with a further benthic species using spiked sediments“ are recommended (European Chemical Bureau, 2003). Here, the ISO 10872 is a suitable alternative. C. elegans has been recommended as a suitable test organisms for testing the toxicity of engineered nanoparticles in complex media, such as soil (Handy et al. 2012).
ISO 10872 is a suitable alternative for testing the toxicity of environmentally hazardous substances in spiked sediments and soils:
Confirmation of chemical concentrations in the test medium is performed by partner contract laboratories.